Journal:

Article Title: Interleukin-1 homologues IL-1F7b and IL-18 contain functional mRNA instability elements within the coding region responsive to lipopolysaccharide

doi: 10.1042/BJ20040217

Figure Lengend Snippet: A region flanking the instability site in human plasminogen activator inhibitor type 2 mRNA [25] is also present in human IL-1 family members as IL-1F7b, IL-1β IL-18 and IL-1Ra. Alignment was generated by using LALIGN-program (www.ch.embnet.org) to compare two DNA sequences for local similarity.

Article Snippet: The monoclonal antibodies against human IL-18 (MAB318) and against His 6 -tagged proteins (MAB050) were purchased from R&D Systems (Minneapolis, MN, U.S.A.).

Techniques: Generated

Journal:

Article Title: Interleukin-1 homologues IL-1F7b and IL-18 contain functional mRNA instability elements within the coding region responsive to lipopolysaccharide

doi: 10.1042/BJ20040217

Figure Lengend Snippet: (A) Freshly isolated human monocytes were stimulated or not with LPS (10 μg/ml) for 4 h. After co-staining against IL-1F7b and Il-18, the cells were visualized using confocal digital microscopy. Red dye, anti-IL-1F7b; green dye, anti-IL-18; blue dye, nuclear stain. (B) Fluorescence was recorded for single cells and the mean counts of intensity (±S.E.M.) for IL-1F7b or IL-18 were calculated by analysing at least 70 individual cells. Statistical differences were calculated by ANOVA; ***P<0.0001.

Article Snippet: The monoclonal antibodies against human IL-18 (MAB318) and against His 6 -tagged proteins (MAB050) were purchased from R&D Systems (Minneapolis, MN, U.S.A.).

Techniques: Isolation, Staining, Microscopy, Fluorescence

Journal:

Article Title: Interleukin-1 homologues IL-1F7b and IL-18 contain functional mRNA instability elements within the coding region responsive to lipopolysaccharide

doi: 10.1042/BJ20040217

Figure Lengend Snippet: RAW264.7 cells stably transfected with human IL-18 cDNA were stimulated with LPS (10 ng/ml). IL-18 mRNA and intracellular protein levels were analysed at the indicated times. (A) Semi-quantitative PCR of one representative experiment. (B) Densitometric analysis of six independent experiments (means±S.E.M.). (C) The lysate of transfected RAW264.7/IL-18 cells before and after treatment with LPS (10 ng/ml) was separated by SDS/PAGE (10% gel) and blotted on to nitrocellulose. The blot was stained using a mAb against human IL-18.

Article Snippet: The monoclonal antibodies against human IL-18 (MAB318) and against His 6 -tagged proteins (MAB050) were purchased from R&D Systems (Minneapolis, MN, U.S.A.).

Techniques: Stable Transfection, Transfection, Real-time Polymerase Chain Reaction, SDS Page, Staining